Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

Clinical Trials & Research
  • 1.

    Evelyn, J. The diary of John Evelyn 1st edn. (Nabu Press, Charleston, 1994).


    Google Scholar
     

  • 2.

    Lau, B. F. et al. The potential of mycelium and culture broth of Lignosus rhinocerotis as substitutes for the naturally occurring sclerotium with regard to antioxidant capacity, cytotoxic effect, and low-molecular-weight chemical constituents. PLoS ONE 9(7), e102509-e (2014).

    ADS 
    Article 
    CAS 

    Google Scholar
     

  • 3.

    Shopana, M., Sudhahar, D. & Anandarajagopal, K. Screening of lignosus rhonocerus extracts as antimicrobial agents against selected human pathogens. J. Pharm. Biomed. Sci. 18(11), 1–4 (2012).


    Google Scholar
     

  • 4.

    Lee, S. S. et al. Anti-inflammatory effect of the sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden, the Tiger Milk mushroom. BMC Compl. Altern Med. 14(1), 359 (2014).

    Article 

    Google Scholar
     

  • 5.

    Johnathan, M., Gan, S. H., Ezumi, M. F. W., Faezahtul, A. H. & Nurul, A. A. Phytochemical profiles and inhibitory effects of Tiger Milk mushroom (Lignosus rhinocerus) extract on ovalbumin-induced airway inflammation in a rodent model of asthma. BMC Compl. Altern. Med. 16, 167 (2016).

    CAS 
    Article 

    Google Scholar
     

  • 6.

    Wong, K.-H., Lai, C. K. M. & Cheung, P. C. K. Immunomodulatory activities of mushroom sclerotial polysaccharides. Food Hydrocolloids 25(2), 150–158 (2011).

    CAS 
    Article 

    Google Scholar
     

  • 7.

    Sillapachaiyaporn, C. & Chuchawankul, S. HIV-1 protease and reverse transcriptase inhibition by tiger milk mushroom (Lignosus rhinocerus) sclerotium extracts: In vitro and in silico studies. J. Tradit. Compl. Med. 10(4), 396–404 (2020).

    Article 

    Google Scholar
     

  • 8.

    Lee, S. S., Tan, N. H., Fung, S. Y., Pailoor, J. & Sim, S. M. Evaluation of the sub-acute toxicity of the sclerotium of Lignosus rhinocerus (Cooke), the Tiger Milk mushroom. J. Ethnopharmacol. 138(1), 192–200 (2011).

    PubMed 
    Article 

    Google Scholar
     

  • 9.

    Cox, M. J., Ege, M. J. & von Mutius, E. Challenges, impact and the future. Lung Microbiome. 83, 240 (2019).

    Article 

    Google Scholar
     

  • 10.

    Soriano, J. B. et al. Prevalence and attributable health burden of chronic respiratory diseases, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet Respir. Med. 8(6), 585–596 (2020).

    Article 

    Google Scholar
     

  • 11.

    Maurer, M. A. et al. Glycosylation of human IGA directly inhibits influenza a and other sialic-acid-binding viruses. Cell Rep. 23(1), 90–99 (2018).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 12.

    Wang, Z. et al. Enhanced SARS-CoV-2 neutralization by dimeric IgA. Sci. Transl. Med. 13(577), eabf1555 (2021).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 13.

    Sterlin, D. et al. IgA dominates the early neutralizing antibody response to SARS-CoV-2. Sci. Transl. Med. 13(577), eabd223 (2021).

    Article 
    CAS 

    Google Scholar
     

  • 14.

    Tiernan, C., Lyons, M., Comyns, T., Nevill, A.M., Warrington, G. Salivary IgA as a predictor of upper respiratory tract infections and relationship to training load in elite rugby union players. J. Strength Condition. Res. 34(3) (2020).

  • 15.

    Garth, J., Barnes, J.W., Krick, S. Targeting cytokines as evolving treatment strategies in chronic inflammatory airway diseases. Int. J. Mol. Sci. 19(11) (2018)

  • 16.

    Aghasafari, P., George, U. & Pidaparti, R. A review of inflammatory mechanism in airway diseases. Inflamm Res. 68(1), 59–74 (2019).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 17.

    Pizzino, G. et al. Oxidative Stress: Harms and Benefits for Human Health. Oxid. Med. Cell Longev. 2017, 8416763 (2017).

    PubMed 
    PubMed Central 

    Google Scholar
     

  • 18.

    Yang, W. & Omaye, S. T. Air pollutants, oxidative stress and human health. Mutation Res. Genetic Toxicol. Environ. Mutagen. 674(1), 45–54 (2009).

    CAS 
    Article 

    Google Scholar
     

  • 19.

    Bortey-Sam, N. et al. Oxidative stress and respiratory symptoms due to human exposure to polycyclic aromatic hydrocarbons (PAHs) in Kumasi, Ghana. Environ. Pollut. 228, 311–320 (2017).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 20.

    Lee, J. S., Shin, J. H., Hwang, J. H., Baek, J. E. & Choi, B. S. Malondialdehyde and 3-nitrotyrosine in exhaled breath condensate in retired elderly coal miners with chronic obstructive pulmonary disease. Saf Health Work. 5(2), 91–96 (2014).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 21.

    Baraldi, E. et al. 3-Nitrotyrosine, a marker of nitrosative stress, is increased in breath condensate of allergic asthmatic children. Allergy 61(1), 90–96 (2006).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 22.

    He, L. et al. Malondialdehyde in nasal fluid: a biomarker for monitoring asthma control in relation to air pollution exposure. Environ. Sci. Technol. 54(18), 11405–11413 (2020).

    ADS 
    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 23.

    Jin, H. et al. Smoking, COPD, and 3-nitrotyrosine levels of plasma proteins. Environ. Health Perspect. 119(9), 1314–1320 (2011).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 24.

    Lıu, X. et al. 8-Hydroxy-2’-deoxyguanosine as a biomarker of oxidative stress in acute exacerbation of chronic obstructive pulmonary disease. Turk. J. Med. Sci. 49(1), 93–100 (2019).

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar
     

  • 25.

    Cao, C. et al. Smoking-promoted oxidative DNA damage response is highly correlated to lung carcinogenesis. Oncotarget 7(14), 18919–18926 (2016).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 26.

    Okamoto, T. et al. Detection of protein-bound 3-nitrotyrosine in the plasma of pediatric patients with severe ARDS and avian influenza virus infection. ADC Lett. Infect. Dis. Control. 6(2), 46–50 (2019).


    Google Scholar
     

  • 27.

    Saito, T. et al. Nasal symptom questionnaire: our proposed scoring system and prognostic factors in chronic rhinosinusitis. ORL J. Otorhinolaryngol. Relat. Spec. 80(5–6), 296–306 (2018).

    PubMed 
    Article 

    Google Scholar
     

  • 28.

    Lappalainen, U., Whitsett, J. A., Wert, S. E., Tichelaar, J. W. & Bry, K. Interleukin-1beta causes pulmonary inflammation, emphysema, and airway remodeling in the adult murine lung. Am. J. Respir. Cell Mol. Biol. 32(4), 311–318 (2005).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 29.

    Pease, J. E. & Sabroe, I. The role of interleukin-8 and its receptors in inflammatory lung disease: implications for therapy. Am. J. Respir. Med. 1(1), 19–25 (2002).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 30.

    Piccioli, P. & Rubartelli, A. The secretion of IL-1β and options for release. Semin. Immunol. 25(6), 425–429 (2013).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 31.

    Brusselle, G. G., Provoost, S., Bracke, K. R., Kuchmiy, A. & Lamkanfi, M. Inflammasomes in respiratory disease: from bench to bedside. Chest 145(5), 1121–1133 (2014).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 32.

    Kolb, M., Margetts, P. J., Anthony, D. C., Pitossi, F. & Gauldie, J. Transient expression of IL-1beta induces acute lung injury and chronic repair leading to pulmonary fibrosis. J. Clin. Invest. 107(12), 1529–1536 (2001).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 33.

    Edwards, M. R., Mukaida, N., Johnson, M. & Johnston, S. L. IL-1β induces IL-8 in bronchial cells via NF-κB and NF-IL6 transcription factors and can be suppressed by glucocorticoids. Pulm. Pharmacol. Ther. 18(5), 337–345 (2005).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 34.

    Muhamad, S. A. et al. Intranasal administration of Lignosus rhinocerotis (Cooke) Ryvarden (Tiger Milk mushroom) extract attenuates airway inflammation in murine model of allergic asthma. Exp. Ther. Med. 17(5), 3867–3876 (2019).

    PubMed 
    PubMed Central 

    Google Scholar
     

  • 35.

    Choi, I. W. et al. TNF-alpha induces the late-phase airway hyperresponsiveness and airway inflammation through cytosolic phospholipase A(2) activation. J. Allergy Clin. Immunol. 116(3), 537–543 (2005).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 36.

    Chang, C. S. et al. Gamma-linolenic acid inhibits inflammatory responses by regulating NF-kappaB and AP-1 activation in lipopolysaccharide-induced RAW 264.7 macrophages. Inflammation 33(1), 46–57 (2010).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 37.

    Furse, R., Rossetti, R., Seiler, C. & Zurier, R. Oral administration of gammalinolenic acid, an unsaturated fatty acid with anti-inflammatory properties, modulates interleukin-1beta production by human monocytes. J. Clin. Immunol. 22, 83–91 (2002).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 38.

    Fricker, M. & Gibson, P. G. Macrophage dysfunction in the pathogenesis and treatment of asthma. Eur. Respir. J. 50(3), 1700196 (2017).

    PubMed 
    Article 
    CAS 

    Google Scholar
     

  • 39.

    Flori, H. et al. A prospective investigation of interleukin-8 levels in pediatric acute respiratory failure and acute respiratory distress syndrome. Crit. Care 23(1), 128 (2019).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 40.

    Reynolds, C. J. et al. Lung defense through IL-8 carries a cost of chronic lung remodeling and impaired function. Am. J. Respir. Cell Mol. Biol. 59(5), 557–571 (2018).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 41.

    Dietz de Loos, D. et al. Prevalence of chronic rhinosinusitis in the general population based on sinus radiology and symptomatology. J. Allergy Clin. Immunol. 143(3), 1207–1214 (2019).

    PubMed 
    Article 

    Google Scholar
     

  • 42.

    Hastan, D. et al. Chronic rhinosinusitis in Europe—an underestimated disease. A GA2 LEN study. Allergy 66(9), 1216–1223 (2011).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 43.

    Ivker RS. Chapter 17—chronic sinusitis. In: Rakel D, editor. Integrative medicine (4th Ed.). Elsevier; 2018. p. 157–69.e2.

  • 44.

    Lee, M. L., Tan, N. H., Fung, S. Y., Tan, C. S. & Ng, S. T. The Antiproliferative Activity of sclerotia of Lignosus rhinocerus (Tiger Milk Mushroom). Evid.-Based Compl. Alternat. Med. 2012, 697603 (2012).

    CAS 

    Google Scholar
     

  • 45.

    Klimek, L. et al. Visual analogue scales (VAS): Measuring instruments for the documentation of symptoms and therapy monitoring in cases of allergic rhinitis in everyday health care: Position Paper of the German Society of Allergology (AeDA) and the German Society of Allergy and Clinical Immunology (DGAKI), ENT Section, in collaboration with the working group on Clinical Immunology, Allergology and Environmental Medicine of the German Society of Otorhinolaryngology, Head and Neck Surgery (DGHNOKHC). Allergo J Int. 26(1), 16–24 (2017).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 46.

    Reyfman, P. A. et al. Defining impaired respiratory health. A paradigm shift for pulmonary medicine. Am. J. Respir. Crit. Care Med. 198(4), 440–446 (2018).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 47.

    Kalhan, R. et al. Lung function in young adults predicts airflow obstruction 20 years later. Am. J. Med. 123(5), 468.e1–7 (2010).

    Article 

    Google Scholar
     

  • 48.

    Agustí, A., Noell, G., Brugada, J. & Faner, R. Lung function in early adulthood and health in later life: a transgenerational cohort analysis. Lancet Respir. Med. 5(12), 935–945 (2017).

    PubMed 
    Article 

    Google Scholar
     

  • 49.

    Lee, M. K. et al. Airway Relaxation effects of water-soluble sclerotial extract from Lignosus rhinocerotis. Front. Pharmacol. 9, 461 (2018).

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar
     

  • 50.

    Pilette, C., Ouadrhiri, Y., Godding, V., Vaerman, J.-P. & Sibille, Y. Lung mucosal immunity: immunoglobulin-A revisited. Eur. Respir. J. 18(3), 571–588 (2001).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 51.

    Yan, H., Lamm, M. E., Björling, E. & Huang, Y. T. Multiple functions of immunoglobulin A in mucosal defense against viruses: an in vitro measles virus model. J. Virol. 76(21), 10972–10979 (2002).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 52.

    Turner, S. E. G., Loosemore, M., Shah, A., Kelleher, P. & Hull, J. H. Salivary IgA as a potential biomarker in the evaluation of respiratory tract infection risk in athletes. J. Allergy Clin. Immunol. 9(1), 151–159 (2021).

    Article 

    Google Scholar
     

  • 53.

    Kubota, A. et al. Reishi mushroom Ganoderma lucidum Modulates IgA production and alpha-defensin expression in the rat small intestine. J. Ethnopharmacol. 214, 240–243 (2018).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 54.

    Li, Y. G. et al. Anti-tumor effects of proteoglycan from Phellinus linteus by immunomodulating and inhibiting Reg IV/EGFR/Akt signaling pathway in colorectal carcinoma. Int. J. Biol. Macromol. 48(3), 511–517 (2011).

    PubMed 
    Article 
    CAS 

    Google Scholar
     

  • 55.

    Johansen, F. E. & Kaetzel, C. S. Regulation of the polymeric immunoglobulin receptor and IgA transport: new advances in environmental factors that stimulate pIgR expression and its role in mucosal immunity. Mucosal. Immunol. 4(6), 598–602 (2011).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 56.

    Scoditti, E., Massaro, M., Garbarino, S. & Toraldo, D. M. Role of diet in chronic obstructive pulmonary disease prevention and treatment. Nutrients 11(6), 1357 (2019).

    CAS 
    PubMed Central 
    Article 
    PubMed 

    Google Scholar
     

  • 57.

    Yap, Y. H. Y. et al. Nutrient composition, antioxidant properties, and anti-proliferative activity of Lignosus rhinocerus Cooke sclerotium. J. Sci. Food Agric. 93(12), 2945–2952 (2013).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 58.

    Yap, H.-Y.Y. et al. Energy and nutritional composition of Tiger milk mushroom (Lignosus tigris Chon S. Tan) sclerotia and the antioxidant activity of its extracts. Int. J. Med. Sci. 11(6), 602–607 (2014).

    PubMed 
    PubMed Central 
    Article 
    CAS 

    Google Scholar
     

  • 59.

    Graf, B. A., Milbury, P. E. & Blumberg, J. B. Flavonols, flavones, flavanones, and human health: epidemiological evidence. J. Med. Food 8(3), 281–290 (2005).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 60.

    Pounis, G. et al. Favorable association of polyphenol-rich diets with lung function: cross-sectional findings from the Moli-sani study. Respir. Med. 136, 48–57 (2018).

    PubMed 
    Article 

    Google Scholar
     

  • 61.

    Pounis, G. et al. Consumption of healthy foods at different content of antioxidant vitamins and phytochemicals and metabolic risk factors for cardiovascular disease in men and women of the Moli–sani study. Eur. J. Clin. Nutr. 67(2), 207–213 (2013).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 62.

    Sartori-Valinotti, J. C., Iliescu, R., Fortepiani, L. A., Yanes, L. L. & Reckelhoff, J. F. Sex differences in oxidative stress and the impact on blood pressure control and cardiovascular disease. Clin. Exp. Pharmacol. Physiol. 34(9), 938–945 (2007).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 63.

    Nadeem, A., Siddiqui, N., Alharbi, N. O. & Alharbi, M. M. Airway and systemic oxidant-antioxidant dysregulation in asthma: a possible scenario of oxidants spill over from lung into blood. Pulm. Pharmacol. Ther. 29(1), 31–40 (2014).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 64.

    Bajpai, J. et al. Study of oxidative stress biomarkers in chronic obstructive pulmonary disease and their correlation with disease severity in north Indian population cohort. Lung India 34(4), 324–329 (2017).

    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 65.

    Ahangarpour, A., Sayahi, M. & Sayahi, M. The antidiabetic and antioxidant properties of some phenolic phytochemicals: a review study. Diabetes Metab. Syndr. 13(1), 854–857 (2019).

    PubMed 
    Article 

    Google Scholar
     

  • 66.

    Nyam, K. L., Chow, C. F., Tan, C. S. & Ng, S. T. Antidiabetic properties of the Tiger’s Milk Medicinal Mushroom, Lignosus rhinocerotis (Agaricomycetes), in streptozotocin-induced diabetic rats. Int. J. Med. Mushrooms 19(7), 607–617 (2017).

    PubMed 
    Article 

    Google Scholar
     

  • 67.

    Auerbach, A. & Hernandez, M. L. The effect of environmental oxidative stress on airway inflammation. Curr. Opin. Allergy Clin. Immunol. 12(2), 133–139 (2012).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 68.

    Shen, Y. et al. Management of airway mucus hypersecretion in chronic airway inflammatory disease: Chinese expert consensus (English edition). Int. J. Chron. Obstruct. Pulmon Dis. 13, 399–407 (2018).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 69.

    Kouadri, A. et al. Involvement of the prion protein in the protection of the human bronchial epithelial barrier against oxidative stress. Antioxid. Redox. Signal. 31(1), 59–74 (2019).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 70.

    Wiegman, C. H. et al. Oxidative stress-induced mitochondrial dysfunction drives inflammation and airway smooth muscle remodeling in patients with chronic obstructive pulmonary disease. J. Allergy Clin. Immunol. 136(3), 769–780 (2015).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 71.

    Kellner, M. et al. ROS signaling in the pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Adv. Exp. Med. Biol. 967, 105–137 (2017).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 72.

    Kleniewska, P. & Pawliczak, R. The participation of oxidative stress in the pathogenesis of bronchial asthma. Biomed. Pharmacother. 94, 100–108 (2017).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 73.

    Manna, P. & Jain, S. K. Obesity, oxidative stress, adipose tissue dysfunction, and the associated health risks: causes and therapeutic strategies. Metab. Syndr. Relat. Disord. 13(10), 423–444 (2015).

    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 74.

    Sutherland, E. R. et al. Cluster analysis of obesity and asthma phenotypes. PLoS ONE 7(5), e36631 (2012).

    ADS 
    CAS 
    PubMed 
    PubMed Central 
    Article 

    Google Scholar
     

  • 75.

    Guideline, I. H. T. Guideline for good clinical practice. J. Postgrad. Med. 47(3), 199–203 (2001).


    Google Scholar
     

  • 76.

    Nikasinovic-Fournier, L. et al. Nasal lavage as a tool for the assessment of upper-airway inflammation in adults and children. J. Lab. Clin. Med. 139(3), 173–180 (2002).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 77.

    Lee, K.-M. et al. A pilot study on the association between job stress and repeated measures of immunological biomarkers in female nurses. Int. Arch. Occup. Environ. Health 83(7), 779–789 (2010).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

  • 78.

    Nakamura, D., Akimoto, T., Suzuki, S. & Kono, I. Daily changes of salivary secretory immunoglobulin A and appearance of upper respiratory symptoms during physical training. J. Sports Med. Phys. Fitness 46(1), 152 (2006).

    CAS 
    PubMed 

    Google Scholar
     

  • 79.

    Bentur, L., Mansour, Y., Brik, R., Eizenberg, Y. & Nagler, R. M. Salivary oxidative stress in children during acute asthmatic attack and during remission. Respir. Med. 100(7), 1195–1201 (2006).

    PubMed 
    Article 

    Google Scholar
     

  • 80.

    Yigla, M., Berkovich, Y. & Nagler, R. M. Oxidative stress indices in COPD—Broncho-alveolar lavage and salivary analysis. Arch. Oral Biol. 52(1), 36–43 (2007).

    CAS 
    PubMed 
    Article 

    Google Scholar
     

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